1,500 jobs available at Continental Tire site

SUMTER, SC (WIS)- Get your resumes together, more than 1,500 jobs are now available at Continental Tire in Sumter County.

Construction is moving right along on the new plant, and it will be completed by the end of the year.

On Friday, the automotive supplier announced it's ready to hire 60 people.

Craig Baartman is project manager and is looking for the new employees.

"This is a game-changer," Baartman said of the new facility. "We believe this is a game-changer for Sumter."

The company is currently looking for skilled workers in engineering and management.

Baartman expects the new 1 million square foot site will bring growth to Highway 521.

"It will attract business, people, tourists," Baartman said.

With a county hovering around an 11.4 percent jobless rate, Baartman believes Continental will do wonders for the local economy. The company is also partnering with local employment agencies and the Central Carolina Technical College to help get candidates trained.

"For those folks out there that are wanting a career, wanting to move on in life, here's a golden opportunity," Baartman said.

Copyright 2012 WIS. All rights reserved.

Source: http://sumter.wistv.com/news/news/53269-1500-jobs-available-continental-tire-site

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Wikipad Gaming Tablet Specifications Revealed | Geeky Gadgets

During an interview with GamesBeat this week, Wikipad the manufacturer and developer of the Wikipad gaming tablet has revealed specifications that will ship with the first devices.

Unfortunately though the new Wikipad gaming tablet will not initially ship with a 3D display, like a prototype that was showcased at this year?s CES exhibition. However the Wikipad will be equipped with a 10.1 inch IPS display offering a resolution of 1,280 x 800 and a 16:10 aspect ratio.

The Wikipad gaming tablet will be powered by a NVIDIA Tegra 3 T30 1.4GHz quad-core processor, supported by 1GB of DDR2 RAM. Together with 16 GB of internal storage and a battery designed to provide 8 hours of video playback, and around 6 hours of continuous gaming pleasure.

When the Wikipad gaming tablet is officially launched later this year it will run Google?s latest Android 4.1 Jelly Bean operating system, and be equipped with both a rear and front facing camera, with 8 megapixels and 2 megapixels respectively.

Source: Venture Beat

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Source: http://www.geeky-gadgets.com/wikipad-gaming-tablet-specifications-revealed-31-07-2012/

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Man who lost hand to alligator charged with unlawful feeding

Weatherholt (Collier County Sheriff's Office)

Talk about adding insult to injury: An Everglades tour guide who lost his hand to an alligator last month was arrested over the weekend, charged with unlawful alligator feeding.

Wallace Weatherholt, a 63-year-old airboat captain, was leading a tour on June 12 when the group encountered a 9-foot alligator. Passengers told the Fort Myers News-Press that Weatherholt "hung a fish over the side of the boat and had his hand at the water's surface when the alligator attacked."

[Related: Pit bull honored as hero]

Wildlife officials then tracked and killed the alligator, according to the paper. They "retrieved Weatherholt's hand from its stomach, but doctors were unable to reattach it."

Weatherholt was charged with unlawful feeding, a second-degree misdemeanor, on Friday. He posted $1,000 and was released from Collier County Jail.

[Related: Bear cubs escape dumpster with a little help]

Feeding alligators is illegal in the state of Florida, where at least 19 people have been cited in the last two years, according to West Palm Beach's WPTV. The charges carry a fine of up to $500 and possible jail time.

Source: http://news.yahoo.com/blogs/sideshow/man-hand-gator-charged-feeding-151153914.html

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Trade Union Boss Says Green Jobs Are Bull****

Another undercover operation by Project Veritas, which is led by investigative video specialist James O?Keefe, has revealed what labor unions truly believe about the billions of dollars that go toward the alleged ?greening? of our energy usage. They?re ?bull****?

Those were the words that came out of the mouth of John Hutchings, a legislator for New York?s Broome County, who is also a construction market representative for the Laborers International Union for upstate New York. He is also an executive officer of the Central New York Labor Federation, AFL-CIO, according to his bio.

Project Veritas actors, who portrayed leaders of a make-believe company called Earth Supply and Renewal, captured the remark in a meeting they obtained with Hutchings and other union leaders to discuss the possibility (again, this was an act) of winning government grants or contracts for their work. The only problem was that Earth Supply and Renewal sold themselves as in the business of carrying out only a very simple task: digging holes, and filling them back in.

?And you guys would be cool with that?? asked a Veritas actor of the union leaders. ?With helping us find funds, even if it?s just ? what we do is just, ?Hey, there?s some dirt to dig,? and put it back in the hole??

Hutchings?s colleague Anthony Tocci, business manager for Laborers International Union-Local 161 on Long Island, replied, ?If people are willing to give you the money, that?s fine.?

Then Hutchings chimed in.

?You know the Green Jobs, Green New York?? he told the actors, referring to a $112-million state weatherization program. ?Between us, a lot of it is bullsh?.

?You know, a lot of that?s going for stuff that?.? Hutchings paused, then raised his hands in the air with a puzzled expression that emphasized the waste of taxpayer dollars and said, ?What are we talking about?? The point: He understood clearly that much of the New York weatherization program dollars were channeled to useless make-work projects.

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Source: http://junkscience.com/2012/07/30/trade-union-boss-says-green-jobs-are-bull/

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Son of Southwest Minn. State coach dies in car crash

CBSSports.com wire reports

July 29, 2012 8:26 PM ET

MARSHALL, Minn. -- Brad Bigler, the men's basketball coach at Southwest Minnesota State University, was in critical condition Sunday after a two-vehicle crash in western Minnesota that killed his 5-month-old son and injured two other relatives.

Bigler, 33, was hospitalized in Sioux Falls, S.D., with several broken ribs and a broken shoulder blade, SMSU athletic director Chris Hmielewski said. Drake Phillip Bigler, the coach's young son, was killed in the crash.

"Obviously it's a tragedy when you lose a young family member," Hmielewski told the Independent of Marshall. "The thoughts and prayers of the entire Mustang family and everybody should go out to the Bigler family as they cope with this situation."

Bigler has been head coach of the SMSU men's basketball program for three years. The Mustangs finished 19-11 last season, winning the Northern Sun Intercollegiate Conference tournament championship and making their third-ever appearance in the Division II tournament.

Bigler's wife, Heather Bigler, was driving in Pope County on Saturday evening when a truck driven by a 38-year-old Starbuck man crossed the center line and struck her vehicle, the Minnesota State Patrol said.

All four people in Bigler's vehicle were hurt. Heather Bigler suffered minor injuries, and her 74-year-old grandmother, Sharon Schuler of Granite Falls, was left in critical condition, authorities said.

The State Patrol said alcohol was detected in the Starbuck man's system, and is suspected as a factor in the crash.

State records show that the driver had been convicted of gross misdemeanor drunken driving in 2005 and for driving under the influence in 2000, according to the Minneapolis Star Tribune. In both cases, the man's blood-alcohol content was greater than 0.20, or 2? times the legal limit in Minnesota.

He also has convictions for reckless driving and speeding.

Before becoming head coach at the university in Marshall, Minn., Bigler previously served as an assistant coach at the school and played for the Mustangs for five seasons.

Hmielewski said the Bigler family sent him a text message early Sunday saying Brad Bigler's condition had improved overnight and that he was awake and coherent.

The crash marked the second tragedy for the Bigler family in just more than a year. Brad Bigler's mother, Diane Bigler-Hagenow, died last summer in a kayaking accident near Sacred Heart.

"We're going to surround the Bigler family with all the love and support they need, and we encourage everyone to do the same," Hmielewski said.

Copyright 2012 by STATS LLC and The Associated Press. Any commercial use or distribution without the express written consent of STATS LLC and The Associated Press is strictly prohibited.

Source: http://feedproxy.google.com/~r/cbssportsline/cb_news/~3/rwD9Ddd0RSw/rss

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Where Lawyers and the Internet Meet: You Need a Technology ...

The internet is a very complicated place. There are web hosting companies, affiliate marketers. There are e-commerce agreements, clickwrap agreements. There?s social media defamation. There is any number of issues surrounding the DNS system, the Domain Name Server system. There?s ICANN which is the Internet Corporation for Assigned Names and Numbers. There?s the registration of domain names which are controlled by registries and are sold through registrars to registrants.

There are subpoena issues in terms of what kind of digital information can you get from internet service providers or ISPs. If you have to identify anonymous people on the internet there?s a whole piece of technology which a lawyer or an attorney has to understand, even to know where to go.

Welcome to Internet Law Radio where we discuss the hottest topics in Internet law.? If you are facing an Internet law issue, cyber law complaint, web site or e-commerce issue, we have an Internet lawyer ready to help.

The internet is a very complicated place.? There are web hosting companies, affiliate marketers. There are e-commerce agreements, clickwrap agreements. There?s social media defamation.? There is any number of issues surrounding the DNS system, the Domain Name Server system.? There?s ICANN which is the Internet Corporation for Assigned Names and Numbers.? There?s the registration of domain names which are controlled by registries and are sold through registrars to registrants.

There are subpoena issues in terms of what kind of digital information can you get from internet service providers or ISPs. If you have to identify anonymous people on the internet there?s a whole piece of technology which a lawyer or an attorney has to understand, even to know where to go.

My name is Internet Law Attorney Enrico Shaefer, and today we?re going to be talking a little bit about this merger between technology and the law.? There used to be, back before the internet, loosely defined technology attorneys, attorneys who understood about software and networks and hardware, and understood enough to be able to lend their legal expertise to clients who existed in those spaces.

But you know what?? The world has changed.? It?s a whole different place, and there?s a lot of business being done on the internet.? The primary calling card for every business is their website, which is a website on a domain name which consumers, and partners, and web visitors go to in order to see who you are and what you do.

So, these assets are incredibly important to virtually every business today. If you?ve got an issue, which somehow deals with your website, your domain name, or with the internet itself, you?re going to need an attorney who not only understands the area of law involved, but perhaps even more importantly understands how the internet works.? A lawyer who does not understand the back-end technology of the internet isn?t going to be able to accomplish results for you as the client.

Attorneys and lawyers today, many of them actually use the internet, right?? They use email.? They get the same spam messages that you do, and they worry about anti-spam laws. But do they really understand how different aspects of the internet and the World Wide Web actually work? The answer is a resoundingly ?no?.? Very few of the older, more experienced lawyers have any experience in internet and technology representation.

So, the most important thing that you need to understand as a client when taking a look at law firms and interviewing attorneys in order to understand who?s going to be able to help you with your internet-based client, with your internet-based problem, is that you need to ask both sides of the equation.? You need to ask your attorneys and your prospective lawyers who you?re taking a look at to hire, not only what the legal issue is, but what is going to have to happen on the technology side in order to accomplish a result?

So, let me give you a couple of examples.? Let?s say that you are a small business or start-up company that is trying to secure a domain name that matches your trademark.? So, there are a number of landing pages out there on domain names which are a good match for your trademark and no one appears to be using those web pages. So, a common question you might have is, ?Well, if I go ahead and register my trademark, can I then go get that domain name??

Well, there are special statues and policies and arbitrations which exist in order to resolve trademark and domain name disputes.? You?re going to need a lawyer who not only understands the law, but understands how the domain name system works, how the Uniform Domain Name Dispute Resolution Policy works, and identify for you your options of securing an unused or a lander page domain name that is going to be the one you?re going to want to launch your business on.

Let?s say you?re a small business or internet start-up company and you?re looking for a lawyer, because someone has defamed you on the internet. Let?s say you?ve been in business for about four years now and you?re growing, but one of your competitors has posted a bogus customer review, alleging that you provide poor customer service and that you didn?t honor your warranties.

Well, if that is posted on a third-party website you need an attorney who not only understands defamation law, but understands how web hosts and website operators work, and what their typical policies are with regards to removing third-party user-generated content that?s alleged to be defamation.

Let me give you another example.? Let?s say you?re a nice little e-commerce business doing say $10 million worth of revenue a year and you?ve been growing very quickly. You?re selling ceiling fans online through a variety of different domain names and websites that your company has built over time.

You do all your original artwork and so all of the different graphics and photographs displaying the different ceiling fans that you have on your website are copyright-owned by you and your company. But your competitors or others start using those original pieces of artwork, those original photographs by your company to display product on eBay, on Amazon, on Buy.com, on other e-commerce sites.

What are your options as a potential copyright owner to have those web pages removed, those accounts blocked by eBay and other vendors and potentially to have the photographs taken down? You?re going to need, not only a copyright law attorney to help you out, but that copyright law attorney is going to have to understand the internet and online space. Otherwise, they will not have any idea what results are achievable and who you can assert leverage against with regards to copyright infringement.

Again, there are special statutes which deal with online copyright infringement and user-generated content.? So, these are the types of things that any good technology and internet lawyer will be able to help you with.

So, when you go out and you list all your requirements on Google and you get those search returns and you find an attorney who you think is going to be good, make sure you take a look at what their technology and internet background is in addition to the substantive area of law that may apply to your specific situation. My name is Internet Law Attorney Enrico Shaefer. We?ll see you next time.

You?ve been listening to Internet Law Radio.? Whether you are facing a domain name, intellectual property or a complex litigation issue, we have an Internet law attorney ready to answer your questions.

Source: http://www.traverselegal.com/internet-law/internet-law/where-lawyers-and-the-internet-meet-you-need-a-technology-lawyer-who-understands-the-internet/

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Source: http://imogendsouzaa.posterous.com/where-lawyers-and-the-internet-meet-you-need

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Health Tip: If You've Broken a Toe

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Source: http://news.yahoo.com/health-tip-youve-broken-toe-110417349.html

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Why Sally Ride's sexuality really matters

America's first woman in space blazed a trail for equality in the sciences thanks to her sex and her sexuality

My girlfriend was the one who pointed out to me that Sally Ride had a female partner.

I was a mess on Monday when I learned that Ride, who became the first American woman to fly in space in 1983, had died. I had no idea she was even sick ? at her request, NASA had kept her 17-month battle with pancreatic cancer secret.

Ride was one of my childhood heroes. I dressed as her for Halloween when I was aged eight and my lifelong passion for space was first budding. Ride's legacy is mostly one of inclusion: bringing more women into science, encouraging girls to think they can do anything. She was a living example of how to crush gender stereotypes, even as she dodged sexist questions from the media. Her first flight on the space shuttle Challenger was before I was born. Thanks to her, I grew up in a world where my sex was not a barrier to the stars.

It runs even deeper for me, as a science writer. When I found out she had double majored in physics and English in college, I felt an intense feeling of identification ? she was like me. I too loved both science and words, and felt a bit of tension about it ? but if the first American woman in space could cross that line, I could too.

And I felt that same sense of recognition when my girlfriend read me this line from Ride's obituary, that she was "survived by her partner of 27 years, Tam O'Shaughnessy." I was elated. Again, Sally Ride was someone like me.

This is why I think Ride's sexuality matters. If her gender matters for diversity reasons, her sexuality matters too, for the same reason.

Role models only work if you see yourself reflected in them. When young people imagine their possible futures ? what career to pursue, where to live, who to spend time with ? one of the most important questions in deciding what path to take is, "Will I find people like me there?"

Psychologists call this "ambient belonging", and it's been shown to have an effect on things like college students' choice of subjects. If you feel that your gender, race, sexuality or love of science fiction sets you apart, then seeing people like you in a certain field will make you more likely to go into it. And the reverse is also true.

Which makes things complicated when it comes to someone like Ride. She was neither out nor deeply in the closet, it seems. Ride doesn't appear to have kept her partnership with O'Shaughnessy particularly secret. They worked together and wrote books together. Would they have legally married if they could? We may never know. But Ride's sex was visible in a way that her sexuality wasn't. She didn't need to put much effort into hiding it for us to overlook it.

Even when you're actively looking, it can be hard to find reflections of yourself in the world. I know that it's not just me. The March meeting of the American Physical Society held the first-ever session on sexual and gender diversity issues in physics this year.

One of the organisers, Elena Long of Kent State University, had searched for resources for LGBT+ physicists ? or even just evidence that others existed ? and found nothing (LGBT stands for lesbian, gay, bisexual and transgender, while the plus sign includes other sexual orientations or gender identities including intersexed, queer, questioning, asexual or pansexual).

One of the main conclusions of the meeting was that having role models who are open about their sexuality, and having a support network was necessary for LGBT+ physicists to feel safe and comfortable in their departments ? and crucial to a successful career.

"I think showing successful academics who are out is an important way to dispel the notion that being out precludes or limits one's professional opportunities," wrote one anonymous attendee.

"There are other people like me who are further in their careers," wrote another. "I know a number of queer students or allies that are students, but I have no visible, contemporary queer scientist role models."

Now we have Sally Ride.?????????

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Enjoyment Furnishings: Leisure Pantry shelves as well as Consoles

Posted by aar2u0zpca on 4 hours ago

There?s a large selection associated with leisure furnishings accessible, which include entertainment cabinets along with consoles which could hold not only massive flat-screen Television sets, but in addition the whole list of top audio system and also the bass speaker woofer, and also all the other hi-fi as well as online games gear you?ve ? together with your ipod connect linked up to the TV or hi-fi speakers.

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This is the short report on the enjoyment pantry shelves as well as games consoles obtainable from an array of United states household furniture manufacturers. It can be by no means extensive, nevertheless offers a excellent selection of solutions offered by three of America?s greater known cupboard producers.

Stickley Home furniture

Gustav Stickley and also their family ended up innovators in most of the methods utilized in U . s . home furniture making, along with he herself was a forerunner inside the U . s . Craftsman style which in turn designed from the United kingdom Martial arts as well as Crafts Movements. Amid these methods ended up the particular extensive use of 1 / 4 sawn oak, the initial centre publish building in which displayed the particular flecks and sun rays of one fourth sawn maple in all four facets, and also a good available kind of construction that will shown the particular jointing methods employed.

Stickley provides numerous entertainment household furniture options, ranging from a straightforward endure to your 68 " TV SET console using space for about 6 containers as well as DIGITAL VIDEO DISC storage space parts. A number of are fully available, although along with additional types it can be hidden at the rear of closed display case doors. Your Stickley trend of TELEVISION consoles comes in a selection of woodlands as well as finishes to fit your getting out of home furniture.

Basically Amish

Basically Amish concentrates on straightforward hand-crafted hard wood furnishings, even though additionally offering an increased level of modification. The particular Just Amish entertainment home furniture contains TELEVISION games cabinets using place for the TELEVISION SET, as well as cabinets and compartments for different elements and also video games consoles.

Your firm also provides complete entertainment pantry shelves that will expand for you to 128 ins, and supply area regarding loudspeakers, Dvd videos, Cds and video games consoles. That they can even be made to blend any TV SET console together with display cabinets as well as ample storage space cabinets and also storage.

Just Amish home furniture is steadily constructed with a range of forest, finishes along with colors. You might be almost specific to identify a wooden as well as finish to check an individual active family room furnishings. The business additionally hand-crafts TV agencies for ones room. Each part of Merely Amish furniture is done exclusively for an individual, and is followed even though make together with your identify onto it.

The particular Customized Shoppe

Similar to those people above, The Custom made Shoppe household furniture can be 100% Manufactured in The us, along with hand-crafted through 100% wood. Both the resources and also the quality are usually with a quite high standard, along with each piece can be individually designed for every customer. Not just that, but if you cannot see what you would like in the catalog it can be created merely that you can your own personal design and style.

The particular Customized Shoppe offers a complete section on its web site to be able to leisure household furniture. They feature a variety, even though company?s enjoyment units are generally especially amazing. A number of these tend to be consumer models, so good that they can ended up maintained in the directory. Allow me to share merely a pair of examples of these:

The actual Kari Home theater

This really is fitted using what is known as your ?Reversica? device. This particular TELEVISION cupboard consists of a pair of screen products either part of your TELEVISION console. The actual gaming console by itself includes 2 doors sometimes aspect involving a collection of shelves what is the best sits the flat-screen console in which moves one hundred eighty certifications to expose one more display area as well as bookshelves ? whichever you want. This kind of any fabulous-looking show unit right up until TELEVISION SET moment, if the key element will be rotated in order to expose the television. It is a optimum regarding 108W a 96H by 31. 5D inches wide.

The Raymond Plasma Pick up

This specific is similar to the aforementioned, using 2 show units sometimes side of your screen device. Simply, in this case the actual plasma or even DIRECTED Television set can be raised from the reduced display case by means of a new rc generator. When straight down, a lovely 3-shelf exhibit product is seen, so when up the TELEVISION can be seen. Your measurements from the aspects of this specific unit are generally varying, along with a maximum of 126W by 96H by 25. 5D throughout in ..

That may purchase TV SET lifts which remotely increase a flat-screen Television set by the end of the mattress. There are lots of other designs of entertainment furniture offered via National home furniture manufacturers, and also they?re only a tiny variety what?s obtainable. We were holding selected to offer you a perception regarding what is achievable, and many leisure cabinets and consoles will certainly adapt to a single or some other of the standard models.

Nevertheless, keep in mind that in case you are not able to come across some thing that suits you, you are able to also have your own amusement furnishings made to your own personal design with a organization like the Tailor made Shoppe. This specific organization is merely an example, and also under no circumstances really the only National home furniture company that may offer this specific service.

Source: http://www.flippingpad.com/profiles/19531-aar2u0zpca/blogs/25688-enjoyment-furnishings-leisure-pantry-shelves-as-well-as-consoles

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Push the Limits in a Powered Paraglider

Think back to when you were young and you would lie on the grass, stare up at the sky and wonder what it would be like to fly among the many clouds. You don't need to wonder anymore with a powered paraglider! These incredible machines are so easy to set up, take down, and fly that anyone can do it! Whether you are a adolescent, or past retirement age this extreme sport is ageless, and has something to provide for everyone!

One of the best things about the powered paraglider is how extremely light weight they are. Some of them as light as 54 pounds! That means there's no need for a crew of guys to help you load or unload you machines, or to have a special trailer to transport it. Just throw it in the back of your car or truck and you are ready to roll!

One thing that isn't required, but is definitely advised is powered paragliding training courses. Since there is no license needed to fly this piece of equipment it means that anyone can go out purchase one and be in the air in the same day. With training become familiar with the specifics of your machine, procedures for flying, and different safety features. It really is crucial that you learn from a trained professional so that it minimizes the danger and maximizes the safety for you and those around you. Courses are usually a matter of 5-7 days long, so it's not like months of training prior to getting to fly your machine; it's a matter of days.

Just to be sure everything is covered, with a powered paraglider you can expect:

- It's an ageless sport for anybody trying to find a new adventure - They are safe and easy to operate - Extremely light weight and compact for storing and hauling - Training courses are available at a low affordable price to ensure safety and knowledge of the machine - No license required to fly - More money in your pocket!

Powered paragliding offers so much in one little machine, what's not to love? People often think back and say 'I wish I would have tried this' or 'I should've done that'. Don't wait another moment to be left wishing on what you should have done. Seize the day and buy a powered paraglider today! You will have nothing but outstanding stories that you can share for years to come.

Source: http://www.artipot.com/articles/1314320/push-the-limits-in-a-powered-paraglider.htm

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PFT: Jones-Drew will be a no-show at camp

Getty Images

To no surprise, Seahawks running back Marshawn Lynch has pleaded not guilty to DUI charges in Oakland, California.? To even less surprise, his lawyer believes that it?s going to take time to resolve the case.

?We will prove that Marshawn was not under the influence when he was pulled over on the evening in question,? lawyer Ivan Golde said, via the San Francisco Chronicle.? ?There is much investigation that needs to be done in this case in that there are many questions about what occurred that are unanswered at this time.? It is going to take some time to unravel everything and, when we do, I am very confident that Marshawn will be exonerated?completely.?

Regardless of whether such an extensive and detailed investigation is needed in order to determine whether Lynch?s blood-alcohol concentration equaled or exceeded the legal limit of 0.08 percent, a delayed resolution ensures that Lynch can?t be suspended in 2012, because he can?t be suspended until the case is resolved.

Even then, Lynch may not be suspended, despite a prior history of two off-field incidents.? As we?ve previously explained, DUIs fall under the substance-abuse policy, not the personal-conduct policy.? For a first-offense DUI (and Lynch is a first-time DUI offender), the penalty ordinarily is a fine in the amount of two game checks.

That said, if Lynch?s camp is confident there won?t be a suspension, he?d be wise to take his alcohol-free medicine in 2012, not 2013.? With a base salary of $4 million this year and $7 million next year, the two-game-check fine would cost $352,941.17 more next year than it would cost this year.

(That said, the Seahawks can always renegotiate his deal and slash his base salary, a fairly easy way to reduce the impact of fines tied to game checks.)

Regardless, even though Golde has created the impression that he wants to move quickly by moving the arraignment from August 15 to Thursday, look for Golde to deftly tap the breaks whenever and however he can.

Source: http://profootballtalk.nbcsports.com/2012/07/26/jones-drew-will-be-a-no-show/related/

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Climate concerns

Friday, July 27, 2012

For decades, scientists have known that the effects of global climate change could have a potentially devastating impact across the globe, but Harvard researchers say there is now evidence that it may also have a dramatic impact on public health.

As reported in a paper published in the July 27 issue of Science, a team of researchers led by James G. Anderson, the Philip S. Weld Professor of Atmospheric Chemistry, are warning that a newly-discovered connection between climate change and depletion of the ozone layer over the U.S. could allow more damaging ultraviolet (UV) radiation to reach the Earth's surface, leading to increased incidence of skin cancer.

In the system described by Anderson and his team, water vapor injected into the stratosphere by powerful thunderstorms converts stable forms of chlorine and bromine into free radicals capable of transforming ozone molecules into oxygen. Recent studies have suggested that the number and intensity of such storms are linked to climate changes, Anderson said, which could in turn lead to increased ozone loss and greater levels of harmful UV radiation reaching the Earth's surface, and potentially higher rates of skin cancer.

"If you were to ask me where this fits into the spectrum of things I worry about, right now it's at the top of the list," Anderson said. "What this research does is connect, for the first time, climate change with ozone depletion, and ozone loss is directly tied to increases in skin cancer incidence, because more ultraviolet radiation is penetrating the atmosphere."

Unfortunately, Anderson said, we don't know how this process will evolve over time.

"We don't know what the development of this has been ? we don't have measurements of this deep convective injection of water into the stratosphere that go back in time," Anderson said.

"But the best guide for the evolution of this is to look at the research that connects climate change with severe storm intensity and frequency, and it's clear that there is a developing scientific case that the addition of carbon dioxide to the atmosphere is increasing climate change, and in turn driving severe storm intensity and frequency."

While it's impossible to know how many skin cancer cases may be related to ozone depletion over the U.S., the link between ozone loss and increased incidence of the disease has been extensively studied, Anderson said.

"There has been a major effort by the medical community to define the relationship between decreases in ozone and the subsequent increases in skin cancer," he said. "The answer is quite clear ? if you multiply the fractional decrease in ozone protection by about three, you get the increase in skin cancer incidence. There are 1 million new skin cancer cases in the U.S. annually ? it's the most common form of cancer, and it's one that's increasing in spite of all the medical research devoted to it."

But it isn't only humans who have to worry about the effects of increased UV radiation.

Many crops, particularly staple crops grown for human consumption ? such as wheat, soybeans and corn ? could suffer damage to their DNA, Anderson said.

Ironically, Anderson said, the discovery that climate change might be driving ozone loss happened virtually by accident.

Though they had worked since the mid-1980s to investigate ozone depletion in the Arctic and Antarctic, by the early-2000s, Anderson's team had turned their attention to climate studies. In particular, they were working to understand how the convective clouds ? updrafts that cause storms to build high into the sky ? contribute to the creation of cirrus clouds.

"It was in the process of looking at that mechanism that we came to this unexpected observation ? that the convective clouds in these storm systems over the U.S. are reaching far deeper into the stratosphere that we ever expected," Anderson said.

While earlier tests performed in the Arctic had demonstrated that water vapor was a key component in creating the "free-radical" compounds that break down ozone, Anderson said the latest finding is much more troubling, because it suggests the process can happen at much higher temperatures than initially suspected.

"The bottom line is that if you increase the water vapor concentration, you actually increase the threshold temperature for executing this chemical conversion ? from the stable forms of chlorine to the free radical form," Anderson said. "If the amount of water vapor and the temperature over the U.S. satisfies the conditions for rapid conversion of inorganic chlorine to this free-radical form, we've got a real problem, because the chemistry is identical to what we previously demonstrated is taking place over the Arctic."

Also surprising, he added, was the realization that, to throw water vapor high into the atmosphere, storms needn't be unusually large.

"We have hundreds of measurements world-wide addressing the photochemical structure controlling ozone, but only a limited number of flights over the U.S. in summer," he said. "The flights were studying average storms over the middle-west, and of the 20 observations we made over the U.S., about half demonstrated significant penetration into the stratosphere," he said.

The next step in the research, Anderson said, is to conduct a series of tests to confirm whether the free-radical form of chlorine and bromine are present in the stratosphere at significantly elevated levels in the presence of convectively-injected water vapor.

"In my mind, this is not just a broad public health issue," Anderson said. "This is about actually being able to step out into the sunlight ? it's about your children and your children's health. Of course, we don't know how rapidly the frequency and intensity of these storms will increase, so we can't place a time scale on this problem, but the core issue here is quite straightforward and simple, because we understand this chemistry."

###

Harvard University: http://www.harvard.edu

Thanks to Harvard University for this article.

This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.

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Source: http://www.labspaces.net/122112/Climate_concerns______

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Fear prompts gun sales, panic after Colo. massacre

Randy Hodges holds a firearm at the Gun Vault in High Point N.C. Monday July 23, 2012. Issues of gun sales, especially assault rifles and large amounts of ammunition, have come into the forefront since a single gunman entered an Aurora, Colo., theater shortly after midnight Friday and began firing indiscriminately at men, women and children. (AP Photo/The Enterprise,Sonny Hedgecock )

Randy Hodges holds a firearm at the Gun Vault in High Point N.C. Monday July 23, 2012. Issues of gun sales, especially assault rifles and large amounts of ammunition, have come into the forefront since a single gunman entered an Aurora, Colo., theater shortly after midnight Friday and began firing indiscriminately at men, women and children. (AP Photo/The Enterprise,Sonny Hedgecock )

Sen. Frank Lautenberg, D-N.J., center, leads a news conference on Capitol Hill in Washington, Tuesday, July 24, 2012, to criticize the sale of high-capacity magazines for assault rifles that are sold to the public. He is joined by Sen. Robert Menendez, D-N.J., left, and Rep. Carolyn McCarthy, D-N.Y., right. A previous federal ban on high-capacity ammunition magazines was allowed to lapse in 2004. (AP Photo/J. Scott Applewhite)

Sen. Frank Lautenberg, D-N.J. gestures during a news conference on Capitol Hill in Washington, Tuesday, July 24, 2012, to criticize the sale of high-capacity magazines for assault rifles that are sold to the public. A previous federal ban on high-capacity ammunition magazines was allowed to lapse in 2004. (AP Photo/J. Scott Applewhite)

Sen. Frank Lautenberg, D-N.J., center, leads a news conference on Capitol Hill in Washington, Tuesday, July 24, 2012, to criticize the sale of high-capacity magazines for assault rifles that are sold to the public. From left are, Rep. Diana DeGette, D-Colo., Sen. Robert Menendez, D-N.J., Lautenberg, and Rep. Carolyn McCarthy, D-N.Y. A previous federal ban on high-capacity ammunition magazines was allowed to lapse in 2004. (AP Photo/J. Scott Applewhite)

DENVER (AP) ? Firearms sales are surging in the wake of the Colorado movie theater massacre as buyers express fears that anti-gun politicians may use the shootings to seek new restrictions on owning weapons.

In Colorado, the site of Friday's shooting that killed 12 and injured dozens of others, gun sales jumped in the three days that followed. The state approved background checks for 2,887 people who wanted to purchase a firearm ? 25 percent more than the average Friday to Sunday period in 2012 and 43 percent more than the same interval the week prior.

Dick Rutan, owner of Gunners Den in suburban Arvada, Colo., said requests for concealed-weapon training certification "are off the hook." His four-hour course in gun safety, required for certification for a concealed-weapons permit in Colorado, has drawn double the interest since Friday.

"What they're saying is: They want to have a chance. They want to have the ability to protect themselves and their families if they are in a situation like what happened in the movie theater," Rutan said.

Day-to-day gun sales frequently fluctuate, but the numbers also look strong outside of Colorado, too.

Seattle's home county, King, saw nearly twice as many requests for concealed pistol licenses than the same timeframe a year ago. Florida recorded 2,386 background checks on Friday, up 14 percent from the week before. Oregon sales on Friday and Saturday were up 11 percent over the month prior. Four days of checks in California were up 10 percent month-to-month.

During the past decade, June and July have consistently been the slowest months for gun sales, according to FBI data.

Jay Wallace, who owns Adventure Outdoors in Smyrna, Ga., found that his sales on Saturday were up 300 percent from the same day a year ago ? making it one of the best Saturdays his business has ever had. He said customers are often afraid when there's a gun-related tragedy that some lawmakers might try and push through an anti-gun agenda.

"We shouldn't let one sick individual make us forget and lose sight of freedoms in this country," Wallace said.

Authorities have said that the suspected Colorado shooter, James Holmes, methodically stockpiled weapons and explosives at work and home in recent months. He purchased thousands of rounds of ammunition and a shotgun, a semi-automatic rifle and two Glock pistols, authorities said.

On Friday, clad in head-to-toe combat gear, he burst into a midnight showing of "The Dark Knight Rises," tossed gas canisters into the crowd and opened fire. The shooting killed 12 people and wounded dozens of others.

Police in the Denver suburb of Aurora say Holmes also booby-trapped his apartment. Holmes is now in solitary confinement at a local jail.

The rise in gun sales reflects but one of the anxieties created by the shootings. Since the massacre, there have been reports of chaos at movie theaters, apparently sparked by misunderstandings or careless words.

A confrontation with an intoxicated man in an Arizona theater caused about 50 people to flee, authorities said. A southern California man was arrested after authorities say he made allusions to the Aurora massacre after the movie didn't start on time. In New Jersey, a showing of "Batman" was canceled after someone stood up during the movie, opened an emergency exit and then returned to their seat.

About 90 minutes into a Monday night showing of "Batman" in Santa Monica, Calif., shrieks from some girls sent about two dozen people sprinting for the exit. It turned out that a large man with a backpack was actually not a threat and was simply having a medical problem.

"This was nothing, and yet it startled us and rattled us so much," said moviegoer Paria Sadighi.

Nationally, the shootings have triggered a fierce debate over gun control and whether government has a role in reining in the ownership of firearms.

Gun sales often fluctuate based on news events, especially whenever people think the passage of more restrictive gun laws is imminent. Sales spiked following the election of President Barack Obama, when weapons enthusiasts expressed fear that the Democrat might curtail gun rights. FBI figures also show background checks for handgun sales jumped in Arizona following the shooting of U.S. Rep. Gabrielle Giffords in 2011.

"It's not uncommon for us to see spikes in requests for concealed pistol licenses when there's a significant gun-related tragedy," said Sgt. Cindi West of the King County sheriff's office in Washington state.

Some Democratic lawmakers in Congress cited the shooting as evidence of the need for tougher gun control laws ? particularly a ban on high-capacity ammunition magazines. Congress, however, hasn't passed strict legislation in more than a decade, and leaders in Washington show no sign of bringing up such measures any time soon.

___

Associated Press writer Mike Baker can be reached on Facebook: http://on.fb.me/HiPpEV

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/386c25518f464186bf7a2ac026580ce7/Article_2012-07-25-US-Colorado-Shooting-Gun-Sales/id-4f9fe390a9cb4e71ac2ec8d4de37736f

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Lady Gaga Sends Best Wishes to Robsten

Count Lady Gaga among the Robsten fans who have not given up hope.

During yesterday's frenzy of gossip over Kristen Stewart, Robert Pattinson and the former's fling with Rupert Sanders, Gaga sent out the following Tweet:

"Geez this kirstew rob stuff is brutal. Makes me sad the way press acts. Hope they're ok."

The pop star wrote that message earlier in the day, however, making it unclear if she was aware of the photos that soon leaked out proving Stewart and Sanders did, indeed, hook up. Or of Stewart's eventual admission.

Since then, the Snow White and the Huntsman director has also apologized and owned up to his actions, while millions of Twilight Saga fans wonder about the fate of Rob and Kristen.

Some of them are also reacting in far less measured tones than Gaga.

Source: http://www.thehollywoodgossip.com/2012/07/lady-gaga-sends-best-wishes-to-robsten/

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Concentra health - HEALTH, BEAUTY & FITNESS

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SGMA Fitness Industry Brief, July 25

Listed below are some of the highlights of the 2012 edition of SGMA?s Tracking the Fitness Movement in the US.
*At the bottom are the top 5 fittest US cities, see if you can guess what they are.

SILVER SPRING, MD ? July 25, 2012 ? There are strong signs that the U.S. fitness industry is vibrant and on the upswing. According to the Sporting Goods Manufacturers Association?s (SGMA) 2012 edition of Tracking the Fitness Movement, there are a number of trends that are driving interest in fitness activities ? both at home and in the health clubs:
1. 18 out of the 30 fitness and exercise activities covered in this report had some increase in participation from 2010 to 2011;
2. The new aerobic activity known as boot-camp style cross training has attracted 7.7 million participants;
3. More than 50 million Americans are using a treadmill;
4. The number of runners/joggers is up 7% from 2010 to 2011; and
5. Nearly 60% of all new fitness participants are women.

?For the legions of Americans who work out, exercise at a health club, or utilize a home gym, they understand the value of fitness in their daily lives ? personally and professionally,? said SGMA President Tom Cove. ?By getting everybody in this country on the same ?fitness? page, we will reduce the burden on the health care system, reduce absenteeism in the classroom, improve productivity in the work force, and enhance the quality of everybody?s lives. It?s an issue which is affecting all aspects of American life ? the private sector, the public sector, academia, and even the military. And SGMA is busy on Capitol Hill supporting legislation that will play a role in getting America fit and healthy.?

Notable News on Fitness:
No Place Like Home. The top five fitness activities which people tend to do more at home than in the health clubs are: (1) home gym exercise, (2) stretching, (3) other exercise to music, (4) low impact aerobics, and (5) yoga.

What?s ?Hot? at Health Clubs. The top five fitness activities which people tend to do more in the gym than at home are: (1) rowing machine, (2) weight/resistance machines, (3) stationary cycling (group), (4) stair-climbing machine, and (5) abdominal machine/device.

Popular Purchases. In the $4.49 billion (at wholesale) fitness industry, the two fitness machine categories which generated the most sales in 2011 were treadmills ($1.146 billion) and elliptical machines ($1.056 billion).

The Fitness Indicators. The five most popular fitness activities in the U.S. are (1) walking for fitness, (2) treadmills, (3) running/jogging, (4) using hand weights, and (5) working out on weight/resistance machines.

Fittest Cities. The top five cities in the U.S. with the highest rates of individual activity (per capita) are: (1) San Francisco/Oakland/San Jose, (2) San Diego, (3) Las Vegas, (4) Austin, and (5) Birmingham (Alabama).

Ultimate Updates

Source: http://www.ultimatesportsequipment.com/3359/sgma-fitness-industry-brief-july-25/

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Mortgage Rates Drop Even Further; Which refinancing option is best ...

By Robert Fishel, on July 25th, 2012

Every borrower?s situation is different. My goal is to provide various options/loan programs that are available to meet the borrower?s needs. When considering a refinance, the following are typical situations borrowers face:

Are you refinancing primarily to lower your rate and monthly payments? Your best option might be a low fixed-rate loan. Maybe you have a fixed-rate mortgage now with a higher rate, or maybe you have an ARM ? adjustable rate mortgage ? where the interest rate varies. Even if it?s low now, unlike your ARM, when you qualify for a fixed-rate mortgage you lock that low rate in for the life of your loan. This is especially a good idea if you don?t think you?ll be moving within the next five years or so. On the other hand, if you do see yourself moving within the next few years, an ARM with a low initial rate might be the best way to lower your monthly payment.

Are you refinancing primarily to cash out some home equity? Maybe you want to pay for home improvements, pay your child?s college tuition bill or possibly payoff other debt, e.g., credit cards, high interest rate loans, etc. Then you?ll want to qualify for a loan for more than the balance remaining on your current mortgage. If you?ve had your current mortgage for a number of years and/or have a mortgage whose interest rate is higher, you may be able to do this without increasing your monthly payment.

Do you want to build up home equity more quickly, and pay off your mortgage sooner? Consider refinancing with a shorter-term loan, such as a 15-year mortgage. Your payments will be higher than with a longer-term loan, but in exchange, you will pay substantially less interest and will build up equity more quickly. If you have had your current 30-year mortgage for a number of years and the loan balance is relatively low, you may be able to do this without increasing your monthly payment ? you may even be able to save!

Whatever the situation, the low interest rates we are currently seeing in the market place could provide an opportunity to save money, provide some liquidity (cash out) or payoff your home at a much faster pace.

MORTGAGE INTEREST RATES for July 25, 2012:

• Conventional 30-Year Fixed 3.500%/ 3.553% APR
• Conventional 15-Year Fixed 2.875%/ 2.969% APR
• Conventional 5/1 ARM 2.625%/ 3.347% APR
• FHA/VA 30 Year Fixed 3.25%/ 3.302% APR
• Jumbo 5/1 ARM 2.750%/ 2.422% APR
• Jumbo 15 yr Fixed 3.375%/ 3.630% APR
• Jumbo 30 yr Fixed 4.750%/ 5.010% APR

*The above mortgage rates are based upon an 80% LTV, o/o single family with FICO scores of 720.

Paramount Mortgage is a locally owned Mortgage Banker celebrating our 41^st year. Great rates and programs are secondary to what is most desired in a lender relationship: Integrity, Communication and Customer Satisfaction. Be to check out our website: www.paramountmortgage.com

For more information or if you have questions on mortgage rates you may contact me by phone at my direct line, (314) 372-4319, email at rfishel@paramountmortgage.com or you can visit our company website at http://www.paramountmortgage.com.

Related posts:

1. Mortgage rates drop; Twenty Two Percent Increase in Refinancing
2. Average mortgage rates set new record low
3. Refinancing Homeowners Reduced Debt In Fourth Quarter
4. Mortgage Rate Update;?The Benefits of ARM?s
5. Mortgage Rate Update;?Why Refinance?

Source: http://realestateconsumernews.com/financing/mortgage-rates-drop-even-further-which-refinancing-option-is-best-for-you/

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Polar bears' ancient roots pushed way back

Genetic blueprints suggest the animals arose millions of years ago

Web edition : Monday, July 23rd, 2012

The polar bear has been around for a surprisingly long time. A new analysis of its DNA suggests that Ursus maritimus split from the brown bear between 4 million and 5 million years ago ? around the same time when, some scientists believe, the Arctic?s thick sea ice first formed.

With such old origins, the creature must have weathered extreme shifts in climate, researchers report online July 23 in the Proceedings of the National Academy of Sciences. Simulations of how the DNA changed over time suggest that polar bear populations rose and fell with the temperature. After thriving during cooler times between 800,000 and 600,000 years ago, the bears seem to have suffered a genetic bottleneck and crashed after a warmer period that started about 420,000 years ago.

Whether the Arctic icon will survive today?s rapid warming remains an open question. ?Even if this species has for sure experienced dramatic climatic changes before, that does not mean it?s safe today,? says Charlotte Lindqvist, an evolutionary biologist at the University at Buffalo in New York.

Lindqvist and colleagues had previously dated the emergence of the polar bear to no more than 150,000 years ago (SN: 3/27/10, p. 14). But that research relied on a single fossil containing mitochondrial DNA, genetic material inherited from the mother. In April, the origin was pushed back to 600,000 years ago by Frank Hailer, an evolutionary biologist at the Biodiversity and Climate Research Centre in Frankfurt (SN: 5/19/12, p. 12). His team examined more than 9,000 letters in nuclear DNA (derived from both parents) of modern bears.

The new study provides an even clearer picture, says Lindqvist, because it looks at the entire genetic blueprints of 23 modern polar bears. ?Lindqvist and colleagues spelled out more than 2.5 billion letters in the creatures? nuclear DNA and identified more than 13 million points of comparison.

But the date in the new study was calibrated using the rate at which mutations appear in the DNA of primates, Hailer says. ?They?re assuming that the genetic clock in bears ticks at the same pace as that in primates.?

Even as it establishes an ancient pedigree for the polar bear, the new research blurs the lines between the species and its darker cousins. Isolated brown bears living in Alaska?s Admiralty, Baranof and Chichagof islands share between 5 and 10 percent of their DNA with polar bears, Lindqvist?s team found. A previous study found that ancient brown bears in Ireland also shared much mitochondrial DNA with their comrades to the north.?

So although polar bears and brown bears separated long ago, they seem to get together every now and then for a fling ? perhaps when the weather warms, sending polar bears southward and brown bears northward. And that interbreeding may in itself be a threat.

?If you get more and more hybridization, you may lose whatever makes polar bears special,? says Ceiridwen Edwards, a geneticist at the University of Oxford in England.

Found in: Earth, Genes & Cells and Life

Source: http://www.sciencenews.org/view/generic/id/342484/title/Polar_bears_ancient_roots_pushed_way_back

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New Mac Owners Can Claim Free Mountain Lion Update http://t.co/ubF8BqFd #apple

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The Up To Date program is specifically designed for those users who have recently purchased a Mac that has shipped with OS X 10.7 Lion. Given that users will be charged a $19.99 fee to upgrade to Mountain Lion, Apple very kindly offers those new Mac owners the opportunity to upgrade free of charge. ?

Source: http://www.facebook.com/applebeatch/posts/505245346158600

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Kickstarter: The RollPro III Is A Must-Have GoPro Camera Storage Case

I'm a GoPro fanboy. It's an amazing camera born from an even more amazing American company. But, as any GoPro owner quickly discovers, the camera demands all sorts of accouterments and add-ons. Mine are stored in a large camera bag with different parts haphazardly thrown into a different compartments. It's essentially a grab bag of GoPro parts. Clearly I'm not alone. Riseful's Kickstarter campaign for the RollPro III tackles this problem with an age-old design.

Source: http://feedproxy.google.com/~r/Techcrunch/~3/MpLRkjCMTmY/

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FastCompany: RT @tgraydar: Push Paws: "Game For Cats" iPad App Makers Stop Kitties From Buying Add-Ons http://t.co/tSTirF3v via @FastCompany

Sorry, Readability was unable to parse this page for content.

Source: http://twitter.com/FastCompany/statuses/227566266670514176

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Lemonade stands raise money for cancer

VIRGINIA BEACH, Va. (WAVY) - On July 20 through July 22, many lemonade stands went up around Hampton Roads to raise money for childhood cancer at CHKD.

Lauren Dubois, 9, spend her day raising money for the annual Anthem LemonAid fundraiser. When Dubois was younger, she received help from the hospital for treatment of a stomach illness.

Her and her family raised more than $200 selling lemonade and cookies Sunday. Photojournalist Aaron Kurtz visited Lauren's Lemonade stand in Virginia Beach on Sunday to see how well her stand was doing.

Source: http://www.wavy.com/dpp/news/local_news/va_beach/lemonade-stands-raise-money-for-cancer

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Colorado Teacher Admits To Having Her Students Baby - The ...

Colorado Spanish teacher Rhonda Eisenberg, 44, was arrested on charges of sexually assaulting a male student. An anonymous tip to the Greeley, Colorado police department landed the high school teacher in handcuffs and eventually behind bars., Find Out More, here, http://www.online-pets-store.com/elgg/blog/view/7361/printable-career-assessment-tests, Adventure, Check Out Your URL
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Lafayette teacher arrested for sex with student ? nola.com LAFAYETTE, La. AP A Lafayette High School teacher is accused of having a sexual relationship with a 17-year-old female student at the school Blaine Peltier, 30, was arrested Thursday. He has resigned his position with the Lafayette Parish School pornennete, Template, Flags, http://www.burracomaniaonline.com/elgg/blog/view/23675/travel-team-by-mike-lupica-quiz, Personality
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New genomic sequencing method enables 'smarter' anaysis of individual cells

Public release date: 22-Jul-2012
[ | E-mail | Share ]

Contact: Rachel Steinhardt
rsteinhardt@licr.org
212-450-1582
Ludwig Institute for Cancer Research

Applications include studying single cells that form malignant tumors

July 22, 2012, New York, NY and Stockholm, Sweden Only by viewing a Seurat painting at close range can you appreciate the hidden complexities of pointillism small, distinct dots of pure color applied in patterns to form an image from a distance. Similarly, biologists and geneticists have long sought to analyze profiles of genes at the single cell level but technology limitations have only allowed a view from afar until now.

Research published in the July 22 issue of Nature Biotechnology, shows for the first time that a novel genomic sequencing method called Smart-Seq can help scientists conduct in-depth analyses of clinically relevant single cells. Smart-Seq has many possible applications, including helping scientists to better understand the complexities of tumor development. This is vitally important as many clinically important cells exist only in small numbers and require single cell analysis. The study was conducted by a team of researchers from the Ludwig Institute for Cancer Research, the Karolinska Institutet in Sweden, the University of California, San Diego and Illumina Inc.

"While our results are preliminary, we showed that it is possible to do studies of individual, clinically relevant cells," says biomedical scientist Rickard Sandberg, researcher at the Ludwig Institute for Cancer Research and principal investigator at the Department of Cell and Molecular Biology, Karolinska Institutet. "Cancer researchers around the world will now be able to analyze these cells more systematically to enable them to produce better methods of diagnosis and therapy in the future."

Previous research showed that it is common for one gene to give rise to several forms of the same protein through different cut-and-paste configurations of its raw copy. The phenomenon, known as splicing, means that cells from the same tissue are not so homogenous as previously thought.

The research team has now taken its study a step further and developed a method for the complete mapping of the gene expression of individual cells. In showing which genes are active, it is now possible to accurately describe and study differences in gene expression between individual cells from the same tissue.

"Scientists have been waiting for a long time for such a method to come along, but technical limitations have made it difficult to produce a sufficiently sensitive and robust method," says Dr. Sandberg. "The method has several areas of applications including cancer research where it can be used to study which cell types form cancer tumors in individual patients."

In the study, scientists studied tumor cells in the blood system of a patient with recurring malignant melanoma. Once they had identified the tumor cells in a regular blood test, the team used Smart-Seq to analyze their gene expression. By using this method, researchers could show that the tumor cells had activated many important membrane proteins that are understood to be responsible for their ability to evade the body's monitoring system and spread in the blood or lymph.

###

The study was conducted with the support of several funding bodies, including the European Research Council, the Swedish Research Council, the Foundation for Strategic Research, the ke Wiberg Foundation and the National Institutes of Health (NIH).

Publication: 'Full-Length mRNA-Seq from single cell levels of RNA and individual circulating tumor cells', Daniel Ramskld, Shujun Luo, Yu-Chieh Wang, Robin Li, Qiaolin Deng, Omid R. Faridani, Gregory A. Daniels, Irina Khrebtukova, Jeanne F. Loring, Louise C. Laurent, Gary P. Schroth and Rickard Sandberg, Nature Biotechnology, online publication 22 July 2012.

About Karolinska Institute

Karolinska Institutet is one of the world's leading medical universities. It accounts for over 40 percent of the medical academic research conducted in Sweden and offers the country's broadest range of education in medicine and health sciences. Since 1901 the Nobel Assembly at Karolinska Institutet has selected the Nobel laureates in Physiology or Medicine.

About The Ludwig Institute for Cancer Research

LICR is an international non-profit organization committed to improving the understanding and control of cancer through integrated laboratory and clinical discovery. Leveraging its worldwide network of investigators and the ability to sponsor and conduct its own clinical trials, the Institute is actively engaged in translating its discoveries into applications for patient benefit. Since its establishment in 1971, the Institute has expended more than $1.5 billion on cancer research.

For further information please contact the KI press room at +46 8 524 860 77 or Pressinfo@ki.se or visit http://ki.se/pressroom and Rachel Steinhardt, rsteinhardt@licr.org or +1-212-450-1582.

---

[ | E-mail | Share ]

?

AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Public release date: 22-Jul-2012
[ | E-mail | Share ]

Contact: Rachel Steinhardt
rsteinhardt@licr.org
212-450-1582
Ludwig Institute for Cancer Research

Applications include studying single cells that form malignant tumors

July 22, 2012, New York, NY and Stockholm, Sweden Only by viewing a Seurat painting at close range can you appreciate the hidden complexities of pointillism small, distinct dots of pure color applied in patterns to form an image from a distance. Similarly, biologists and geneticists have long sought to analyze profiles of genes at the single cell level but technology limitations have only allowed a view from afar until now.

Research published in the July 22 issue of Nature Biotechnology, shows for the first time that a novel genomic sequencing method called Smart-Seq can help scientists conduct in-depth analyses of clinically relevant single cells. Smart-Seq has many possible applications, including helping scientists to better understand the complexities of tumor development. This is vitally important as many clinically important cells exist only in small numbers and require single cell analysis. The study was conducted by a team of researchers from the Ludwig Institute for Cancer Research, the Karolinska Institutet in Sweden, the University of California, San Diego and Illumina Inc.

"While our results are preliminary, we showed that it is possible to do studies of individual, clinically relevant cells," says biomedical scientist Rickard Sandberg, researcher at the Ludwig Institute for Cancer Research and principal investigator at the Department of Cell and Molecular Biology, Karolinska Institutet. "Cancer researchers around the world will now be able to analyze these cells more systematically to enable them to produce better methods of diagnosis and therapy in the future."

Previous research showed that it is common for one gene to give rise to several forms of the same protein through different cut-and-paste configurations of its raw copy. The phenomenon, known as splicing, means that cells from the same tissue are not so homogenous as previously thought.

The research team has now taken its study a step further and developed a method for the complete mapping of the gene expression of individual cells. In showing which genes are active, it is now possible to accurately describe and study differences in gene expression between individual cells from the same tissue.

"Scientists have been waiting for a long time for such a method to come along, but technical limitations have made it difficult to produce a sufficiently sensitive and robust method," says Dr. Sandberg. "The method has several areas of applications including cancer research where it can be used to study which cell types form cancer tumors in individual patients."

In the study, scientists studied tumor cells in the blood system of a patient with recurring malignant melanoma. Once they had identified the tumor cells in a regular blood test, the team used Smart-Seq to analyze their gene expression. By using this method, researchers could show that the tumor cells had activated many important membrane proteins that are understood to be responsible for their ability to evade the body's monitoring system and spread in the blood or lymph.

###

The study was conducted with the support of several funding bodies, including the European Research Council, the Swedish Research Council, the Foundation for Strategic Research, the ke Wiberg Foundation and the National Institutes of Health (NIH).

Publication: 'Full-Length mRNA-Seq from single cell levels of RNA and individual circulating tumor cells', Daniel Ramskld, Shujun Luo, Yu-Chieh Wang, Robin Li, Qiaolin Deng, Omid R. Faridani, Gregory A. Daniels, Irina Khrebtukova, Jeanne F. Loring, Louise C. Laurent, Gary P. Schroth and Rickard Sandberg, Nature Biotechnology, online publication 22 July 2012.

About Karolinska Institute

Karolinska Institutet is one of the world's leading medical universities. It accounts for over 40 percent of the medical academic research conducted in Sweden and offers the country's broadest range of education in medicine and health sciences. Since 1901 the Nobel Assembly at Karolinska Institutet has selected the Nobel laureates in Physiology or Medicine.

About The Ludwig Institute for Cancer Research

LICR is an international non-profit organization committed to improving the understanding and control of cancer through integrated laboratory and clinical discovery. Leveraging its worldwide network of investigators and the ability to sponsor and conduct its own clinical trials, the Institute is actively engaged in translating its discoveries into applications for patient benefit. Since its establishment in 1971, the Institute has expended more than $1.5 billion on cancer research.

For further information please contact the KI press room at +46 8 524 860 77 or Pressinfo@ki.se or visit http://ki.se/pressroom and Rachel Steinhardt, rsteinhardt@licr.org or +1-212-450-1582.

---

[ | E-mail | Share ]

?

AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.

Source: http://www.eurekalert.org/pub_releases/2012-07/lifc-ngs071912.php

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